^DATABASE = GeoMiame
!Database_name = Gene Expression Omnibus (GEO)
!Database_institute = NCBI NLM NIH
!Database_web_link = http://www.ncbi.nlm.nih.gov/geo
!Database_email = geo@ncbi.nlm.nih.gov
^SERIES = GSE194234
!Series_title = RNA sequencing of primary Sjögren’s syndrome neutrophils
!Series_geo_accession = GSE194234
!Series_status = Public on Jan 25 2022
!Series_submission_date = Jan 23 2022
!Series_last_update_date = Jan 25 2022
!Series_summary = Objective Neutrophils and aberrant NETosis have been implicated in the pathogenesis of diverse autoimmune diseases, however, their roles in primary Sjögren’s syndrome (pSS) remain unclear. We aimed to reveal the potential roles of neutrophils and neutrophil extracellular traps (NETs) in this study.
!Series_summary = Methods pSS patients were enrolled according to the corresponding diagnostic criteria. NETosis markers were measured in plasma and small salivary gland using ELISA and immunofluorescence. The gene signatures of neutrophils were assessed by RNA-Seq and RT-PCR. Reactive oxygen species (ROS), mitochondrial ROS (mitoROS) production and JC-1 was measured by flow cytometry.
!Series_summary = Results NETosis markers including cell free-DNA (cf-DNA), myeloperoxidase (MPO) in plasma and small salivary gland from pSS patients were significantly higher than healthy controls (HCs) and were associated with disease activity. RNA sequencing and RT-qPCR revealed activated Type I IFN signaling pathway and higher expression of type I interferon related genes in pSS neutrophils. Further stimulating with IFN-α 2a in vitro significantly induced ROS production and JC-1 monomer percentage  in pSS neutrophils.
!Series_summary = Conclusions Our data suggest the involvement of neutrophils and enhanced NETosis in pSS patients. Further mechanism study in vitro revealed that type I IFN activation in pSS neutrophils led to mitochondrial damage and related ROS production which finally result in the generation of NETs.
!Series_overall_design = RNA sequencing of 7 pSS patients and 6 healthy controls
!Series_type = Expression profiling by high throughput sequencing
!Series_contributor = Yu,,Peng
!Series_contributor = Xunyao,,Wu
!Series_contributor = Shulan,,Zhang
!Series_contributor = Yunyun,,Fei
!Series_sample_id = GSM5831329
!Series_sample_id = GSM5831330
!Series_sample_id = GSM5831331
!Series_sample_id = GSM5831332
!Series_sample_id = GSM5831333
!Series_sample_id = GSM5831334
!Series_sample_id = GSM5831335
!Series_sample_id = GSM5831336
!Series_sample_id = GSM5831337
!Series_sample_id = GSM5831338
!Series_sample_id = GSM5831339
!Series_sample_id = GSM5831340
!Series_sample_id = GSM5831341
!Series_contact_name = Yu,,Peng
!Series_contact_email = pengyu180488@126.com
!Series_contact_institute = Peking Union Medical College Hospital
!Series_contact_address = No. 1, Shuaifu yuan
!Series_contact_city = Beijing
!Series_contact_zip/postal_code = 100010
!Series_contact_country = China
!Series_supplementary_file = ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE194nnn/GSE194234/suppl/GSE194234_mRNA_Diff_gene.FPKM.xlsx
!Series_platform_id = GPL11154
!Series_platform_taxid = 9606
!Series_sample_taxid = 9606
!Series_relation = BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA799777
^PLATFORM = GPL11154
!Platform_title = Illumina HiSeq 2000 (Homo sapiens)
!Platform_geo_accession = GPL11154
!Platform_status = Public on Nov 02 2010
!Platform_submission_date = Nov 02 2010
!Platform_last_update_date = Mar 27 2019
!Platform_technology = high-throughput sequencing
!Platform_distribution = virtual
!Platform_organism = Homo sapiens
!Platform_taxid = 9606
!Platform_contact_name = ,,GEO
!Platform_contact_country = USA
!Platform_data_row_count = 0
^SAMPLE = GSM5831329
!Sample_title = Healthy control 1
!Sample_geo_accession = GSM5831329
!Sample_status = Public on Jan 25 2022
!Sample_submission_date = Jan 23 2022
!Sample_last_update_date = Jan 25 2022
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = healthy control_Neutrophils
!Sample_organism_ch1 = Homo sapiens
!Sample_taxid_ch1 = 9606
!Sample_characteristics_ch1 = cell type: Neutrophils
!Sample_characteristics_ch1 = treatment: none
!Sample_characteristics_ch1 = subject status: healthy control
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = Total RNA of neutrophils was extracted using TRIzol reagent (Invitrogen, USA) and stored at -80℃ for subsequent RNA sequencing (Novogene, China).
!Sample_extract_protocol_ch1 = A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
!Sample_extract_protocol_ch1 = First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 µl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
!Sample_data_processing = Raw data of fastq format were firstly processed through in-house per scripts and clean data were obtained by removing reads containing adapter, reads containing poly-N and low-quality reads.
!Sample_data_processing = HTseq v0.6.0 was used to count the reads numbers mapped to each gene.
!Sample_data_processing = Differential expression analysis was performed using DESeq2 R package (1.10.1).
!Sample_data_processing = Genome_build: mm9
!Sample_data_processing = Supplementary_files_format_and_content: FPKM values for each Sample
!Sample_platform_id = GPL11154
!Sample_contact_name = Yu,,Peng
!Sample_contact_email = pengyu180488@126.com
!Sample_contact_institute = Peking Union Medical College Hospital
!Sample_contact_address = No. 1, Shuaifu yuan
!Sample_contact_city = Beijing
!Sample_contact_zip/postal_code = 100010
!Sample_contact_country = China
!Sample_instrument_model = Illumina HiSeq 2000
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN25209020
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13876462
!Sample_supplementary_file_1 = NONE
!Sample_series_id = GSE194234
!Sample_data_row_count = 0
^SAMPLE = GSM5831330
!Sample_title = Healthy control 2
!Sample_geo_accession = GSM5831330
!Sample_status = Public on Jan 25 2022
!Sample_submission_date = Jan 23 2022
!Sample_last_update_date = Jan 25 2022
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = healthy control_Neutrophils
!Sample_organism_ch1 = Homo sapiens
!Sample_taxid_ch1 = 9606
!Sample_characteristics_ch1 = cell type: Neutrophils
!Sample_characteristics_ch1 = treatment: none
!Sample_characteristics_ch1 = subject status: healthy control
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = Total RNA of neutrophils was extracted using TRIzol reagent (Invitrogen, USA) and stored at -80℃ for subsequent RNA sequencing (Novogene, China).
!Sample_extract_protocol_ch1 = A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
!Sample_extract_protocol_ch1 = First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 µl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
!Sample_data_processing = Raw data of fastq format were firstly processed through in-house per scripts and clean data were obtained by removing reads containing adapter, reads containing poly-N and low-quality reads.
!Sample_data_processing = HTseq v0.6.0 was used to count the reads numbers mapped to each gene.
!Sample_data_processing = Differential expression analysis was performed using DESeq2 R package (1.10.1).
!Sample_data_processing = Genome_build: mm9
!Sample_data_processing = Supplementary_files_format_and_content: FPKM values for each Sample
!Sample_platform_id = GPL11154
!Sample_contact_name = Yu,,Peng
!Sample_contact_email = pengyu180488@126.com
!Sample_contact_institute = Peking Union Medical College Hospital
!Sample_contact_address = No. 1, Shuaifu yuan
!Sample_contact_city = Beijing
!Sample_contact_zip/postal_code = 100010
!Sample_contact_country = China
!Sample_instrument_model = Illumina HiSeq 2000
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN25209019
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13876463
!Sample_supplementary_file_1 = NONE
!Sample_series_id = GSE194234
!Sample_data_row_count = 0
^SAMPLE = GSM5831331
!Sample_title = Healthy control 3
!Sample_geo_accession = GSM5831331
!Sample_status = Public on Jan 25 2022
!Sample_submission_date = Jan 23 2022
!Sample_last_update_date = Jan 25 2022
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = healthy control_Neutrophils
!Sample_organism_ch1 = Homo sapiens
!Sample_taxid_ch1 = 9606
!Sample_characteristics_ch1 = cell type: Neutrophils
!Sample_characteristics_ch1 = treatment: none
!Sample_characteristics_ch1 = subject status: healthy control
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = Total RNA of neutrophils was extracted using TRIzol reagent (Invitrogen, USA) and stored at -80℃ for subsequent RNA sequencing (Novogene, China).
!Sample_extract_protocol_ch1 = A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
!Sample_extract_protocol_ch1 = First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 µl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
!Sample_data_processing = Raw data of fastq format were firstly processed through in-house per scripts and clean data were obtained by removing reads containing adapter, reads containing poly-N and low-quality reads.
!Sample_data_processing = HTseq v0.6.0 was used to count the reads numbers mapped to each gene.
!Sample_data_processing = Differential expression analysis was performed using DESeq2 R package (1.10.1).
!Sample_data_processing = Genome_build: mm9
!Sample_data_processing = Supplementary_files_format_and_content: FPKM values for each Sample
!Sample_platform_id = GPL11154
!Sample_contact_name = Yu,,Peng
!Sample_contact_email = pengyu180488@126.com
!Sample_contact_institute = Peking Union Medical College Hospital
!Sample_contact_address = No. 1, Shuaifu yuan
!Sample_contact_city = Beijing
!Sample_contact_zip/postal_code = 100010
!Sample_contact_country = China
!Sample_instrument_model = Illumina HiSeq 2000
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN25209018
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13876464
!Sample_supplementary_file_1 = NONE
!Sample_series_id = GSE194234
!Sample_data_row_count = 0
^SAMPLE = GSM5831332
!Sample_title = Healthy control 4
!Sample_geo_accession = GSM5831332
!Sample_status = Public on Jan 25 2022
!Sample_submission_date = Jan 23 2022
!Sample_last_update_date = Jan 25 2022
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = healthy control_Neutrophils
!Sample_organism_ch1 = Homo sapiens
!Sample_taxid_ch1 = 9606
!Sample_characteristics_ch1 = cell type: Neutrophils
!Sample_characteristics_ch1 = treatment: none
!Sample_characteristics_ch1 = subject status: healthy control
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = Total RNA of neutrophils was extracted using TRIzol reagent (Invitrogen, USA) and stored at -80℃ for subsequent RNA sequencing (Novogene, China).
!Sample_extract_protocol_ch1 = A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
!Sample_extract_protocol_ch1 = First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 µl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
!Sample_data_processing = Raw data of fastq format were firstly processed through in-house per scripts and clean data were obtained by removing reads containing adapter, reads containing poly-N and low-quality reads.
!Sample_data_processing = HTseq v0.6.0 was used to count the reads numbers mapped to each gene.
!Sample_data_processing = Differential expression analysis was performed using DESeq2 R package (1.10.1).
!Sample_data_processing = Genome_build: mm9
!Sample_data_processing = Supplementary_files_format_and_content: FPKM values for each Sample
!Sample_platform_id = GPL11154
!Sample_contact_name = Yu,,Peng
!Sample_contact_email = pengyu180488@126.com
!Sample_contact_institute = Peking Union Medical College Hospital
!Sample_contact_address = No. 1, Shuaifu yuan
!Sample_contact_city = Beijing
!Sample_contact_zip/postal_code = 100010
!Sample_contact_country = China
!Sample_instrument_model = Illumina HiSeq 2000
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN25209017
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13876465
!Sample_supplementary_file_1 = NONE
!Sample_series_id = GSE194234
!Sample_data_row_count = 0
^SAMPLE = GSM5831333
!Sample_title = Healthy control 5
!Sample_geo_accession = GSM5831333
!Sample_status = Public on Jan 25 2022
!Sample_submission_date = Jan 23 2022
!Sample_last_update_date = Jan 25 2022
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = healthy control_Neutrophils
!Sample_organism_ch1 = Homo sapiens
!Sample_taxid_ch1 = 9606
!Sample_characteristics_ch1 = cell type: Neutrophils
!Sample_characteristics_ch1 = treatment: none
!Sample_characteristics_ch1 = subject status: healthy control
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = Total RNA of neutrophils was extracted using TRIzol reagent (Invitrogen, USA) and stored at -80℃ for subsequent RNA sequencing (Novogene, China).
!Sample_extract_protocol_ch1 = A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
!Sample_extract_protocol_ch1 = First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 µl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
!Sample_data_processing = Raw data of fastq format were firstly processed through in-house per scripts and clean data were obtained by removing reads containing adapter, reads containing poly-N and low-quality reads.
!Sample_data_processing = HTseq v0.6.0 was used to count the reads numbers mapped to each gene.
!Sample_data_processing = Differential expression analysis was performed using DESeq2 R package (1.10.1).
!Sample_data_processing = Genome_build: mm9
!Sample_data_processing = Supplementary_files_format_and_content: FPKM values for each Sample
!Sample_platform_id = GPL11154
!Sample_contact_name = Yu,,Peng
!Sample_contact_email = pengyu180488@126.com
!Sample_contact_institute = Peking Union Medical College Hospital
!Sample_contact_address = No. 1, Shuaifu yuan
!Sample_contact_city = Beijing
!Sample_contact_zip/postal_code = 100010
!Sample_contact_country = China
!Sample_instrument_model = Illumina HiSeq 2000
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN25209016
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13876466
!Sample_supplementary_file_1 = NONE
!Sample_series_id = GSE194234
!Sample_data_row_count = 0
^SAMPLE = GSM5831334
!Sample_title = Healthy control 6
!Sample_geo_accession = GSM5831334
!Sample_status = Public on Jan 25 2022
!Sample_submission_date = Jan 23 2022
!Sample_last_update_date = Jan 25 2022
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = healthy control_Neutrophils
!Sample_organism_ch1 = Homo sapiens
!Sample_taxid_ch1 = 9606
!Sample_characteristics_ch1 = cell type: Neutrophils
!Sample_characteristics_ch1 = treatment: none
!Sample_characteristics_ch1 = subject status: healthy control
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = Total RNA of neutrophils was extracted using TRIzol reagent (Invitrogen, USA) and stored at -80℃ for subsequent RNA sequencing (Novogene, China).
!Sample_extract_protocol_ch1 = A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
!Sample_extract_protocol_ch1 = First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 µl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
!Sample_data_processing = Raw data of fastq format were firstly processed through in-house per scripts and clean data were obtained by removing reads containing adapter, reads containing poly-N and low-quality reads.
!Sample_data_processing = HTseq v0.6.0 was used to count the reads numbers mapped to each gene.
!Sample_data_processing = Differential expression analysis was performed using DESeq2 R package (1.10.1).
!Sample_data_processing = Genome_build: mm9
!Sample_data_processing = Supplementary_files_format_and_content: FPKM values for each Sample
!Sample_platform_id = GPL11154
!Sample_contact_name = Yu,,Peng
!Sample_contact_email = pengyu180488@126.com
!Sample_contact_institute = Peking Union Medical College Hospital
!Sample_contact_address = No. 1, Shuaifu yuan
!Sample_contact_city = Beijing
!Sample_contact_zip/postal_code = 100010
!Sample_contact_country = China
!Sample_instrument_model = Illumina HiSeq 2000
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN25209015
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13876467
!Sample_supplementary_file_1 = NONE
!Sample_series_id = GSE194234
!Sample_data_row_count = 0
^SAMPLE = GSM5831335
!Sample_title = pSS 1
!Sample_geo_accession = GSM5831335
!Sample_status = Public on Jan 25 2022
!Sample_submission_date = Jan 23 2022
!Sample_last_update_date = Jan 25 2022
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = pSS_Neutrophils
!Sample_organism_ch1 = Homo sapiens
!Sample_taxid_ch1 = 9606
!Sample_characteristics_ch1 = cell type: Neutrophils
!Sample_characteristics_ch1 = treatment: none
!Sample_characteristics_ch1 = subject status: primary Sjögren’s syndrome (pSS) patient
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = Total RNA of neutrophils was extracted using TRIzol reagent (Invitrogen, USA) and stored at -80℃ for subsequent RNA sequencing (Novogene, China).
!Sample_extract_protocol_ch1 = A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
!Sample_extract_protocol_ch1 = First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 µl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
!Sample_data_processing = Raw data of fastq format were firstly processed through in-house per scripts and clean data were obtained by removing reads containing adapter, reads containing poly-N and low-quality reads.
!Sample_data_processing = HTseq v0.6.0 was used to count the reads numbers mapped to each gene.
!Sample_data_processing = Differential expression analysis was performed using DESeq2 R package (1.10.1).
!Sample_data_processing = Genome_build: mm9
!Sample_data_processing = Supplementary_files_format_and_content: FPKM values for each Sample
!Sample_platform_id = GPL11154
!Sample_contact_name = Yu,,Peng
!Sample_contact_email = pengyu180488@126.com
!Sample_contact_institute = Peking Union Medical College Hospital
!Sample_contact_address = No. 1, Shuaifu yuan
!Sample_contact_city = Beijing
!Sample_contact_zip/postal_code = 100010
!Sample_contact_country = China
!Sample_instrument_model = Illumina HiSeq 2000
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN25209014
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13876468
!Sample_supplementary_file_1 = NONE
!Sample_series_id = GSE194234
!Sample_data_row_count = 0
^SAMPLE = GSM5831336
!Sample_title = pSS 2
!Sample_geo_accession = GSM5831336
!Sample_status = Public on Jan 25 2022
!Sample_submission_date = Jan 23 2022
!Sample_last_update_date = Jan 25 2022
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = pSS_Neutrophils
!Sample_organism_ch1 = Homo sapiens
!Sample_taxid_ch1 = 9606
!Sample_characteristics_ch1 = cell type: Neutrophils
!Sample_characteristics_ch1 = treatment: none
!Sample_characteristics_ch1 = subject status: primary Sjögren’s syndrome (pSS) patient
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = Total RNA of neutrophils was extracted using TRIzol reagent (Invitrogen, USA) and stored at -80℃ for subsequent RNA sequencing (Novogene, China).
!Sample_extract_protocol_ch1 = A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
!Sample_extract_protocol_ch1 = First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 µl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
!Sample_data_processing = Raw data of fastq format were firstly processed through in-house per scripts and clean data were obtained by removing reads containing adapter, reads containing poly-N and low-quality reads.
!Sample_data_processing = HTseq v0.6.0 was used to count the reads numbers mapped to each gene.
!Sample_data_processing = Differential expression analysis was performed using DESeq2 R package (1.10.1).
!Sample_data_processing = Genome_build: mm9
!Sample_data_processing = Supplementary_files_format_and_content: FPKM values for each Sample
!Sample_platform_id = GPL11154
!Sample_contact_name = Yu,,Peng
!Sample_contact_email = pengyu180488@126.com
!Sample_contact_institute = Peking Union Medical College Hospital
!Sample_contact_address = No. 1, Shuaifu yuan
!Sample_contact_city = Beijing
!Sample_contact_zip/postal_code = 100010
!Sample_contact_country = China
!Sample_instrument_model = Illumina HiSeq 2000
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN25209013
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13876469
!Sample_supplementary_file_1 = NONE
!Sample_series_id = GSE194234
!Sample_data_row_count = 0
^SAMPLE = GSM5831337
!Sample_title = pSS 3
!Sample_geo_accession = GSM5831337
!Sample_status = Public on Jan 25 2022
!Sample_submission_date = Jan 23 2022
!Sample_last_update_date = Jan 25 2022
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = pSS_Neutrophils
!Sample_organism_ch1 = Homo sapiens
!Sample_taxid_ch1 = 9606
!Sample_characteristics_ch1 = cell type: Neutrophils
!Sample_characteristics_ch1 = treatment: none
!Sample_characteristics_ch1 = subject status: primary Sjögren’s syndrome (pSS) patient
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = Total RNA of neutrophils was extracted using TRIzol reagent (Invitrogen, USA) and stored at -80℃ for subsequent RNA sequencing (Novogene, China).
!Sample_extract_protocol_ch1 = A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
!Sample_extract_protocol_ch1 = First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 µl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
!Sample_data_processing = Raw data of fastq format were firstly processed through in-house per scripts and clean data were obtained by removing reads containing adapter, reads containing poly-N and low-quality reads.
!Sample_data_processing = HTseq v0.6.0 was used to count the reads numbers mapped to each gene.
!Sample_data_processing = Differential expression analysis was performed using DESeq2 R package (1.10.1).
!Sample_data_processing = Genome_build: mm9
!Sample_data_processing = Supplementary_files_format_and_content: FPKM values for each Sample
!Sample_platform_id = GPL11154
!Sample_contact_name = Yu,,Peng
!Sample_contact_email = pengyu180488@126.com
!Sample_contact_institute = Peking Union Medical College Hospital
!Sample_contact_address = No. 1, Shuaifu yuan
!Sample_contact_city = Beijing
!Sample_contact_zip/postal_code = 100010
!Sample_contact_country = China
!Sample_instrument_model = Illumina HiSeq 2000
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN25209012
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13876470
!Sample_supplementary_file_1 = NONE
!Sample_series_id = GSE194234
!Sample_data_row_count = 0
^SAMPLE = GSM5831338
!Sample_title = pSS 4
!Sample_geo_accession = GSM5831338
!Sample_status = Public on Jan 25 2022
!Sample_submission_date = Jan 23 2022
!Sample_last_update_date = Jan 25 2022
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = pSS_Neutrophils
!Sample_organism_ch1 = Homo sapiens
!Sample_taxid_ch1 = 9606
!Sample_characteristics_ch1 = cell type: Neutrophils
!Sample_characteristics_ch1 = treatment: none
!Sample_characteristics_ch1 = subject status: primary Sjögren’s syndrome (pSS) patient
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = Total RNA of neutrophils was extracted using TRIzol reagent (Invitrogen, USA) and stored at -80℃ for subsequent RNA sequencing (Novogene, China).
!Sample_extract_protocol_ch1 = A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
!Sample_extract_protocol_ch1 = First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 µl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
!Sample_data_processing = Raw data of fastq format were firstly processed through in-house per scripts and clean data were obtained by removing reads containing adapter, reads containing poly-N and low-quality reads.
!Sample_data_processing = HTseq v0.6.0 was used to count the reads numbers mapped to each gene.
!Sample_data_processing = Differential expression analysis was performed using DESeq2 R package (1.10.1).
!Sample_data_processing = Genome_build: mm9
!Sample_data_processing = Supplementary_files_format_and_content: FPKM values for each Sample
!Sample_platform_id = GPL11154
!Sample_contact_name = Yu,,Peng
!Sample_contact_email = pengyu180488@126.com
!Sample_contact_institute = Peking Union Medical College Hospital
!Sample_contact_address = No. 1, Shuaifu yuan
!Sample_contact_city = Beijing
!Sample_contact_zip/postal_code = 100010
!Sample_contact_country = China
!Sample_instrument_model = Illumina HiSeq 2000
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN25209011
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13876471
!Sample_supplementary_file_1 = NONE
!Sample_series_id = GSE194234
!Sample_data_row_count = 0
^SAMPLE = GSM5831339
!Sample_title = pSS 5
!Sample_geo_accession = GSM5831339
!Sample_status = Public on Jan 25 2022
!Sample_submission_date = Jan 23 2022
!Sample_last_update_date = Jan 25 2022
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = pSS_Neutrophils
!Sample_organism_ch1 = Homo sapiens
!Sample_taxid_ch1 = 9606
!Sample_characteristics_ch1 = cell type: Neutrophils
!Sample_characteristics_ch1 = treatment: none
!Sample_characteristics_ch1 = subject status: primary Sjögren’s syndrome (pSS) patient
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = Total RNA of neutrophils was extracted using TRIzol reagent (Invitrogen, USA) and stored at -80℃ for subsequent RNA sequencing (Novogene, China).
!Sample_extract_protocol_ch1 = A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
!Sample_extract_protocol_ch1 = First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 µl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
!Sample_data_processing = Raw data of fastq format were firstly processed through in-house per scripts and clean data were obtained by removing reads containing adapter, reads containing poly-N and low-quality reads.
!Sample_data_processing = HTseq v0.6.0 was used to count the reads numbers mapped to each gene.
!Sample_data_processing = Differential expression analysis was performed using DESeq2 R package (1.10.1).
!Sample_data_processing = Genome_build: mm9
!Sample_data_processing = Supplementary_files_format_and_content: FPKM values for each Sample
!Sample_platform_id = GPL11154
!Sample_contact_name = Yu,,Peng
!Sample_contact_email = pengyu180488@126.com
!Sample_contact_institute = Peking Union Medical College Hospital
!Sample_contact_address = No. 1, Shuaifu yuan
!Sample_contact_city = Beijing
!Sample_contact_zip/postal_code = 100010
!Sample_contact_country = China
!Sample_instrument_model = Illumina HiSeq 2000
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN25209010
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13876472
!Sample_supplementary_file_1 = NONE
!Sample_series_id = GSE194234
!Sample_data_row_count = 0
^SAMPLE = GSM5831340
!Sample_title = pSS 6
!Sample_geo_accession = GSM5831340
!Sample_status = Public on Jan 25 2022
!Sample_submission_date = Jan 23 2022
!Sample_last_update_date = Jan 25 2022
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = pSS_Neutrophils
!Sample_organism_ch1 = Homo sapiens
!Sample_taxid_ch1 = 9606
!Sample_characteristics_ch1 = cell type: Neutrophils
!Sample_characteristics_ch1 = treatment: none
!Sample_characteristics_ch1 = subject status: primary Sjögren’s syndrome (pSS) patient
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = Total RNA of neutrophils was extracted using TRIzol reagent (Invitrogen, USA) and stored at -80℃ for subsequent RNA sequencing (Novogene, China).
!Sample_extract_protocol_ch1 = A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
!Sample_extract_protocol_ch1 = First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 µl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
!Sample_data_processing = Raw data of fastq format were firstly processed through in-house per scripts and clean data were obtained by removing reads containing adapter, reads containing poly-N and low-quality reads.
!Sample_data_processing = HTseq v0.6.0 was used to count the reads numbers mapped to each gene.
!Sample_data_processing = Differential expression analysis was performed using DESeq2 R package (1.10.1).
!Sample_data_processing = Genome_build: mm9
!Sample_data_processing = Supplementary_files_format_and_content: FPKM values for each Sample
!Sample_platform_id = GPL11154
!Sample_contact_name = Yu,,Peng
!Sample_contact_email = pengyu180488@126.com
!Sample_contact_institute = Peking Union Medical College Hospital
!Sample_contact_address = No. 1, Shuaifu yuan
!Sample_contact_city = Beijing
!Sample_contact_zip/postal_code = 100010
!Sample_contact_country = China
!Sample_instrument_model = Illumina HiSeq 2000
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN25209009
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13876473
!Sample_supplementary_file_1 = NONE
!Sample_series_id = GSE194234
!Sample_data_row_count = 0
^SAMPLE = GSM5831341
!Sample_title = pSS 7
!Sample_geo_accession = GSM5831341
!Sample_status = Public on Jan 25 2022
!Sample_submission_date = Jan 23 2022
!Sample_last_update_date = Jan 25 2022
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = pSS_Neutrophils
!Sample_organism_ch1 = Homo sapiens
!Sample_taxid_ch1 = 9606
!Sample_characteristics_ch1 = cell type: Neutrophils
!Sample_characteristics_ch1 = treatment: none
!Sample_characteristics_ch1 = subject status: primary Sjögren’s syndrome (pSS) patient
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = Total RNA of neutrophils was extracted using TRIzol reagent (Invitrogen, USA) and stored at -80℃ for subsequent RNA sequencing (Novogene, China).
!Sample_extract_protocol_ch1 = A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
!Sample_extract_protocol_ch1 = First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 µl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
!Sample_data_processing = Raw data of fastq format were firstly processed through in-house per scripts and clean data were obtained by removing reads containing adapter, reads containing poly-N and low-quality reads.
!Sample_data_processing = HTseq v0.6.0 was used to count the reads numbers mapped to each gene.
!Sample_data_processing = Differential expression analysis was performed using DESeq2 R package (1.10.1).
!Sample_data_processing = Genome_build: mm9
!Sample_data_processing = Supplementary_files_format_and_content: FPKM values for each Sample
!Sample_platform_id = GPL11154
!Sample_contact_name = Yu,,Peng
!Sample_contact_email = pengyu180488@126.com
!Sample_contact_institute = Peking Union Medical College Hospital
!Sample_contact_address = No. 1, Shuaifu yuan
!Sample_contact_city = Beijing
!Sample_contact_zip/postal_code = 100010
!Sample_contact_country = China
!Sample_instrument_model = Illumina HiSeq 2000
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN25209008
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13876474
!Sample_supplementary_file_1 = NONE
!Sample_series_id = GSE194234
!Sample_data_row_count = 0