^DATABASE = GeoMiame
!Database_name = Gene Expression Omnibus (GEO)
!Database_institute = NCBI NLM NIH
!Database_web_link = http://www.ncbi.nlm.nih.gov/geo
!Database_email = geo@ncbi.nlm.nih.gov
^SERIES = GSE189213
!Series_title = Transcriptional profiling of mouse eosinophils identifies distinct gene signatures following cellular activation
!Series_geo_accession = GSE189213
!Series_status = Public on Dec 04 2021
!Series_submission_date = Nov 19 2021
!Series_last_update_date = Jan 10 2022
!Series_pubmed_id = 34970274
!Series_summary = Purpose: we aimed to characterized the transcriptional profile of mouse eosinophils in response to stimuli that are associated with Type 1 and Type 2 environments
!Series_summary = Methods: Eosinophils were purified from the peritoneal cavity of Il5Tg mice and stimulated with IL-4 (i.e. type 2 activated eosinophils), IFN-γ, and a combination of lipopolysaccharide (LPS, i.e. type 1 activated eosinophils) in the presence of IFN-γ. Thereafter, RNA was obtained and the transcriptome signature following each stimulation was determined using RNA sequencing. Raw sequencing reads were trimmed and filtered using fastp, then aligned to mouse genome assembly (GRCm38) using STAR 2.7.2a. Normalization and differential expression analysis were performed in R 4.0.3 using the package DESeq2. Finally, gene ontology annotations were obtained from Ensembl and pathway graphs were obtained from KEGG.
!Series_summary = Results: RNAseq analysis revealed marked differences, which were driven by the different stimulating conditions
!Series_summary = Conclusion: We demonstrate that eosinophils are polarized to distinct phenotypes following distinct stimuli. These findings contribute to the growing understanding regarding the heterogeneity of eosinophils and their possible roles in different diseases.
!Series_overall_design = RNAseq of eosinophils following activation with stimuli that are associated with Type 1 and Type 2 environments
!Series_type = Expression profiling by high throughput sequencing
!Series_sample_id = GSM5696199
!Series_sample_id = GSM5696200
!Series_sample_id = GSM5696201
!Series_sample_id = GSM5696202
!Series_sample_id = GSM5696203
!Series_sample_id = GSM5696204
!Series_sample_id = GSM5696205
!Series_sample_id = GSM5696206
!Series_sample_id = GSM5696207
!Series_sample_id = GSM5696208
!Series_sample_id = GSM5696209
!Series_sample_id = GSM5696210
!Series_sample_id = GSM5696211
!Series_sample_id = GSM5696212
!Series_sample_id = GSM5696213
!Series_sample_id = GSM5696214
!Series_sample_id = GSM5696215
!Series_sample_id = GSM5696216
!Series_contact_name = Guy,,Shapira
!Series_contact_email = guyspersonal@gmail.com
!Series_contact_institute = Tel Aviv University
!Series_contact_address = Chaim Levanon 30
!Series_contact_city = Tel Aviv
!Series_contact_zip/postal_code = 6997801
!Series_contact_country = Israel
!Series_supplementary_file = ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE189nnn/GSE189213/suppl/GSE189213_RAW.tar
!Series_platform_id = GPL21626
!Series_platform_taxid = 10090
!Series_sample_taxid = 10090
!Series_relation = BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA781989
!Series_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRP346951
^PLATFORM = GPL21626
!Platform_title = NextSeq 550 (Mus musculus)
!Platform_geo_accession = GPL21626
!Platform_status = Public on Mar 22 2016
!Platform_submission_date = Mar 22 2016
!Platform_last_update_date = Jan 15 2019
!Platform_technology = high-throughput sequencing
!Platform_distribution = virtual
!Platform_organism = Mus musculus
!Platform_taxid = 10090
!Platform_contact_name = ,,GEO
!Platform_contact_country = USA
!Platform_data_row_count = 0
^SAMPLE = GSM5696199
!Sample_title = Unstimulaetd eosinophils a
!Sample_geo_accession = GSM5696199
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: unstimulated
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310707
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179852
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696199/suppl/GSM5696199_EosOnly1.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696200
!Sample_title = Unstimulaetd eosinophils b
!Sample_geo_accession = GSM5696200
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: unstimulated
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310708
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179853
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696200/suppl/GSM5696200_EosOnly2.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696201
!Sample_title = Unstimulaetd eosinophils c
!Sample_geo_accession = GSM5696201
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: unstimulated
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310709
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179854
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696201/suppl/GSM5696201_EosOnly3.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696202
!Sample_title = E. coli Stimulated Eosinophils 1
!Sample_geo_accession = GSM5696202
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: E. coli
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310711
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179855
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696202/suppl/GSM5696202_EosE.coli1.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696203
!Sample_title = E. coli Stimulated Eosinophils 2
!Sample_geo_accession = GSM5696203
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: E. coli
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310712
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179856
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696203/suppl/GSM5696203_EosE.coli2.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696204
!Sample_title = E. coli Stimulated Eosinophils 3
!Sample_geo_accession = GSM5696204
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: E. coli
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310713
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179857
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696204/suppl/GSM5696204_EosE.coli3.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696205
!Sample_title = IFNγ Stimulated Eosinophils 1
!Sample_geo_accession = GSM5696205
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: IFN[gamma]
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310714
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179858
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696205/suppl/GSM5696205_EosINFg1.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696206
!Sample_title = IFNγ Stimulated Eosinophils 2
!Sample_geo_accession = GSM5696206
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: IFN[gamma]
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310695
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179859
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696206/suppl/GSM5696206_EosINFg2.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696207
!Sample_title = IFNγ Stimulated Eosinophils 3
!Sample_geo_accession = GSM5696207
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: IFN[gamma]
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310696
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179860
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696207/suppl/GSM5696207_EosINFg3.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696208
!Sample_title = IFNγ+E. coli Stimulated Eosinophils 1
!Sample_geo_accession = GSM5696208
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: IFN[gamma] + E. coli
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310698
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179861
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696208/suppl/GSM5696208_EosINFgE.coli1.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696209
!Sample_title = IFNγ+E. coli Stimulated Eosinophils 2
!Sample_geo_accession = GSM5696209
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: IFN[gamma] + E. coli
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310699
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179862
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696209/suppl/GSM5696209_EosINFgE.coli2.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696210
!Sample_title = IFNγ+E. coli Stimulated Eosinophils 3
!Sample_geo_accession = GSM5696210
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: IFN[gamma] + E. coli
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310701
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179863
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696210/suppl/GSM5696210_EosINFgE.coli3.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696211
!Sample_title = Unstimulaetd eosinophils d
!Sample_geo_accession = GSM5696211
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: unstimulated
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310715
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179864
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696211/suppl/GSM5696211_Eosinophlisonly_1.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696212
!Sample_title = Unstimulaetd eosinophils e
!Sample_geo_accession = GSM5696212
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: unstimulated
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310716
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179865
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696212/suppl/GSM5696212_Eosinophlisonly_2.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696213
!Sample_title = Unstimulaetd eosinophils f
!Sample_geo_accession = GSM5696213
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: unstimulated
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310693
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179866
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696213/suppl/GSM5696213_Eosinophlisonly_3.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696214
!Sample_title = IL-4 Stimulated Eosinophils 1
!Sample_geo_accession = GSM5696214
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: IL-4
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310703
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179867
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696214/suppl/GSM5696214_Eosinophlis_IL4_1.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696215
!Sample_title = IL-4 Stimulated Eosinophils 2
!Sample_geo_accession = GSM5696215
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: IL-4
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310704
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179868
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696215/suppl/GSM5696215_Eosinophlis_IL4_2.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0
^SAMPLE = GSM5696216
!Sample_title = IL-4 Stimulated Eosinophils 3
!Sample_geo_accession = GSM5696216
!Sample_status = Public on Dec 04 2021
!Sample_submission_date = Nov 19 2021
!Sample_last_update_date = Dec 04 2021
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Eosinophils
!Sample_organism_ch1 = Mus musculus
!Sample_taxid_ch1 = 10090
!Sample_characteristics_ch1 = strain: C57BL/6
!Sample_characteristics_ch1 = treatment: IL-4
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
!Sample_data_processing = RNA-Seq data was trimmed using fastp 0.20.1
!Sample_data_processing = RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
!Sample_data_processing = CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
!Sample_data_processing = Genome_build: GRCm38
!Sample_data_processing = Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
!Sample_platform_id = GPL21626
!Sample_contact_name = Guy,,Shapira
!Sample_contact_email = guyspersonal@gmail.com
!Sample_contact_institute = Tel Aviv University
!Sample_contact_address = Chaim Levanon 30
!Sample_contact_city = Tel Aviv
!Sample_contact_zip/postal_code = 6997801
!Sample_contact_country = Israel
!Sample_instrument_model = NextSeq 550
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = RNA-Seq
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN23310705
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX13179869
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5696nnn/GSM5696216/suppl/GSM5696216_Eosinophlis_IL4_3.counts.tsv.gz
!Sample_series_id = GSE189213
!Sample_data_row_count = 0